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aurumtm total rna mini kit  (Bio-Rad)


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    Structured Review

    Bio-Rad aurumtm total rna mini kit
    Aurumtm Total Rna Mini Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 2891 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aurum+total+rna+mini+kit/Aurum+Total+RNA+Mini+Kit/pm42098955-96-32-37
    Average 96 stars, based on 2891 article reviews
    aurumtm total rna mini kit - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Synthesized:

    Article Title: Mitochondrial ETF insufficiency drives neoplastic growth by selectively optimizing cancer bioenergetics
    Article Snippet: .. HCT-116 and NT2197 cells were plated in 6-well dishes and extracted using TRIzol (Ambion) or Aurum Total RNA Mini Kit (Bio-Rad) following the manufacturer’s instructions. cDNA was synthesized from purified RNA using SensiFAST cDNA Synthesis Kit (Bioline), as per the manufacturer’s protocol. .. RT-qPCR was performed using SensiFAST SYBR Lo-ROX kit (Bioline).

    Article Title: FXR and BET signaling orchestrate to protect β cells
    Article Snippet: .. Cell lines were lysed using TRIzol reagent (Ambion, 15596018) and phenol-chloroform-isoamyl alcohol treatment, followed by total RNA extraction using the column of Aurum Total RNA Mini Kit (Bio-Rad, 732-6820) according to the manufacturer’s instructions. cDNA was synthesized from 500 - 1000 ng of total RNA treated with DNase I using the iScript cDNA Synthesis Kit (Bio-Rad, 1708891). .. Real-time quantitative RT-PCR (qPCR) was performed using the iTaqTM Universal SYBR Green Supermix (Bio-Rad, 1725122), and analyses were performed using the ABI7900HT Fast Real-Time PCR System or the ABI Step One Plus Real-Time PCR System.

    Purification:

    Article Title: Mitochondrial ETF insufficiency drives neoplastic growth by selectively optimizing cancer bioenergetics
    Article Snippet: .. HCT-116 and NT2197 cells were plated in 6-well dishes and extracted using TRIzol (Ambion) or Aurum Total RNA Mini Kit (Bio-Rad) following the manufacturer’s instructions. cDNA was synthesized from purified RNA using SensiFAST cDNA Synthesis Kit (Bioline), as per the manufacturer’s protocol. .. RT-qPCR was performed using SensiFAST SYBR Lo-ROX kit (Bioline).

    cDNA Synthesis:

    Article Title: Mitochondrial ETF insufficiency drives neoplastic growth by selectively optimizing cancer bioenergetics
    Article Snippet: .. HCT-116 and NT2197 cells were plated in 6-well dishes and extracted using TRIzol (Ambion) or Aurum Total RNA Mini Kit (Bio-Rad) following the manufacturer’s instructions. cDNA was synthesized from purified RNA using SensiFAST cDNA Synthesis Kit (Bioline), as per the manufacturer’s protocol. .. RT-qPCR was performed using SensiFAST SYBR Lo-ROX kit (Bioline).

    Article Title: FXR and BET signaling orchestrate to protect β cells
    Article Snippet: .. Cell lines were lysed using TRIzol reagent (Ambion, 15596018) and phenol-chloroform-isoamyl alcohol treatment, followed by total RNA extraction using the column of Aurum Total RNA Mini Kit (Bio-Rad, 732-6820) according to the manufacturer’s instructions. cDNA was synthesized from 500 - 1000 ng of total RNA treated with DNase I using the iScript cDNA Synthesis Kit (Bio-Rad, 1708891). .. Real-time quantitative RT-PCR (qPCR) was performed using the iTaqTM Universal SYBR Green Supermix (Bio-Rad, 1725122), and analyses were performed using the ABI7900HT Fast Real-Time PCR System or the ABI Step One Plus Real-Time PCR System.

    Isolation:

    Article Title: The Role of Dopamine Signaling and Neuroinflammation in Age‐Related Cognitive Dysfunction
    Article Snippet: Samples were homogenized in cell disruption lysis buffer (Millipore, MA, USA) using 1.5‐mm zirconium beads (Triple‐Pure) and a BeadBug microtube homogenizer. .. The supernatant was extracted, and RNA isolation was performed according to the manufacturer's protocol for the Aurum Total RNA Mini Kit (Bio‐Rad, CA, USA). .. RNA concentration was quantified using a Nanodrop Lite spectrophotometer (Thermo Fisher Scientific, UK).

    Article Title: FXR and BET signaling orchestrate to protect β cells
    Article Snippet: .. Total RNA was isolated from TRIzol (Ambion, 15596018) treated cell using the Aurum Total RNA Mini Kit (BIO-RAD, 7326820) and treated with DNase I for 30 minutes at room temperature (RT). .. Sequencing libraries preparation and sequencing was performed at Novogene, Inc. Short read sequences were mapped to a UCSC hg38 reference sequence using the RNA-Seq aligner STAR .

    Article Title: The Role of Dopamine Signaling and Neuroinflammation in Age-Related Cognitive Dysfunction.
    Article Snippet: Samples ere homogenized in cell disruption lysis buffer (Millipore, MA, SA) using 1.5-mm zirconium beads (Triple-Pure) and a BeadBug icrotube homogenizer. .. The supernatant was extracted, and NA isolation was performed according to the manufacturer’s rotocol for the Aurum Total RNA Mini Kit (Bio-Rad, CA, USA). .. NA concentration was quantified using a Nanodrop Lite specrophotometer (Thermo Fisher Scientific, UK).

    Article Title: Zinc-redox crosstalk regulates proteostasis in the endoplasmic reticulum.
    Article Snippet: .. mRNAs were isolated using an RNeasy Kit (QIAGEN) or Aurum Total RNA Mini Kit (Bio-Rad) and analyzed by qRT-PCR using PrimePCR assays (Bio-Rad, Supplementary Table 3) and SsoAdvanced Universal SYBR Green Supermix (Bio-Rad). .. The thermal cycle reaction and SYBR Green detection were performed using a CFX96 Touch Deep Well Real-Time PCR Detection System (Bio-Rad).

    RNA Extraction:

    Article Title: FXR and BET signaling orchestrate to protect β cells
    Article Snippet: .. Cell lines were lysed using TRIzol reagent (Ambion, 15596018) and phenol-chloroform-isoamyl alcohol treatment, followed by total RNA extraction using the column of Aurum Total RNA Mini Kit (Bio-Rad, 732-6820) according to the manufacturer’s instructions. cDNA was synthesized from 500 - 1000 ng of total RNA treated with DNase I using the iScript cDNA Synthesis Kit (Bio-Rad, 1708891). .. Real-time quantitative RT-PCR (qPCR) was performed using the iTaqTM Universal SYBR Green Supermix (Bio-Rad, 1725122), and analyses were performed using the ABI7900HT Fast Real-Time PCR System or the ABI Step One Plus Real-Time PCR System.

    Quantitative RT-PCR:

    Article Title: Zinc-redox crosstalk regulates proteostasis in the endoplasmic reticulum.
    Article Snippet: .. mRNAs were isolated using an RNeasy Kit (QIAGEN) or Aurum Total RNA Mini Kit (Bio-Rad) and analyzed by qRT-PCR using PrimePCR assays (Bio-Rad, Supplementary Table 3) and SsoAdvanced Universal SYBR Green Supermix (Bio-Rad). .. The thermal cycle reaction and SYBR Green detection were performed using a CFX96 Touch Deep Well Real-Time PCR Detection System (Bio-Rad).

    SYBR Green Assay:

    Article Title: Zinc-redox crosstalk regulates proteostasis in the endoplasmic reticulum.
    Article Snippet: .. mRNAs were isolated using an RNeasy Kit (QIAGEN) or Aurum Total RNA Mini Kit (Bio-Rad) and analyzed by qRT-PCR using PrimePCR assays (Bio-Rad, Supplementary Table 3) and SsoAdvanced Universal SYBR Green Supermix (Bio-Rad). .. The thermal cycle reaction and SYBR Green detection were performed using a CFX96 Touch Deep Well Real-Time PCR Detection System (Bio-Rad).



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